![]() ![]() (1984) Signal sequences distinguish class II histocompatibility antigen beta chains of different loci. Gustafsson K., Wiman K., Larhammer D., Rask L., and Peterson P. (1982) Allelic polymorphism and complexity of the genes for HLA-DR beta-chains-direct analysis by DNA-DNA hybridization. ![]() (1981) Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of a oligodeoxynucleotide primer. (1988) DNA-RFLP analysis and genotyping of HLADR and DQ antigens. (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. (1979) A rapid alkaline extraction procedure for screening recombinant plasmid DNA. (1970) Calcium dependant bacteriophage DNA infection. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. (1988) A simple saltingout procedure for extracting DNA from human nucleated cells. (1995) Association between an MHC class II allele and clearance of hepatitis B virus in the Gambia. This process is experimental and the keywords may be updated as the learning algorithm improves. These keywords were added by machine and not by the authors. Restriction Fragment Length Polymorphism.This approach may also find application for the identification of polymorphism in new genes within the MHC and perhaps for the nonclassical HLA genes. In this chapter, the basic method for Southern blot and the identification of RFLP will be outlined. Some relatively recent publications have used RFLP for the identification of HLA class II polymorphism in large population studies ( 1). The close correlation found between serology and RFLP pointed to the conserved organization of introns within the major histocompatibility complex (MHC), and the RFLP method quickly established that HLA and disease associations were not due to gross genomic reorganizations (insertions or deletions) in the areas examined. Historically, RFLP identification of HLA class II polymorphism was important at a time when class II antisera were relatively poorly defined and B cell separation for serological typing from peripheral blood was sometimes problematic. As a practical method for the identification of human leukocyte antigen (HLA) class I or II alleles, the restriction fragment length polymorphism (RFLP) approach has almost entirely been superceded by other DNA-based methods, in particular those based on polymerase chain reaction (PCR). ![]()
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